In order to improve the quality and efficiency of railway crew routing plan, the problem of crew routing plan was abstracted as a Multi-Traveling Salesman Problem (MTSP) with single base and balanced travel distance, and a equilibrium factor was introduced to establish a mathematical model aiming at less crew routing time and balanced tasks between sub-crew routings. A dual-strategy ant colony optimization algorithm was proposed for this model. Firstly, a solution space satisfying the space-time constraints was constructed and pheromone concentration was set for the node of the crew section and the continuation path respectively, then the transitional probability of the dual-strategy state was adopted to make the ant traverse all of the crew segments, and finally the sub-crew routings that meet the crew constraint rules were found. The designed model and algorithm were tested by the data of the intercity railway from Guangzhou to Shenzhen. The comparison with the experimental results of genetic algorithm shows that under the same model conditions, the number of crew routing in the crew routing plan generated by double-strategy ant colony optimization algorithm is reduced by about 21.74%, the total length of crew routing is decreased by about 5.76%, and the routing overload rate is 0. Using the designed model and algorithm to generate the crew routing plan can reduce the crew routing time of crew plan, balance the workload and avoid overload routing.